Fig. 3: NLGN3 plays a role in maintaining cancer stem cell properties of GBM cells. | Cell Death & Disease

Fig. 3: NLGN3 plays a role in maintaining cancer stem cell properties of GBM cells.

From: Targeting Wnt/β-catenin-mediated upregulation of oncogenic NLGN3 suppresses cancer stem cells in glioblastoma

Fig. 3

NLGN3, NRXN3 mRNA (A) and DAB2IP (B) expressions were compared between monolayer (2D) and sphere (3D) culture condition. Red and blue bars indicate DAB2IP-high and DAB2IP-low expression cell lines, respectively. Means ± SD; One-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001. C After restoration of NLGN3 expressions in U87MG OE (DAB2IP-high, NLGN3-low) and LN229 OE (DAB2IP-high, NLGN3-low) cells, sphere forming abilities including both numbers and size were compared. Red and blue bars indicate DAB2IP-high and DAB2IP-low expression cell lines, green bar indicates NLGN3 restoration in DAB2IP-high cells, respectively. Means ± SD; One-way ANOVA, ***p < 0.001. Scale bar=100 µm. D After restoration of NLGN3 expressions in U87MG OE and LN229 OE cells, CD133 mRNA expression was compared. Means ± SD; One-way ANOVA, *p < 0.05, ***p < 0.001. E After restoration of NLGN3 expressions in U87MG OE and LN229 OE cells, cells were stained with PE-conjugated CD133 and analyzed by flow cytometry. F U87MG OE and LN229 OE cells were co-transfected with NLGN3 and increment amount of NRXN3, and CD133 mRNA expression was compared. Means ± SD; One-way ANOVA, **p < 0.01, ***p < 0.001.

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