Fig. 2: Neuronal network degeneration in tricultures with microglia and astrocytes, but not neuronal monocultures, is reversible with mitochondria fission inhibitor P110. | Cell Death & Disease

Fig. 2: Neuronal network degeneration in tricultures with microglia and astrocytes, but not neuronal monocultures, is reversible with mitochondria fission inhibitor P110.

From: Mitochondria dysregulation contributes to secondary neurodegeneration progression post-contusion injury in human 3D in vitro triculture brain tissue model

Fig. 2

a Schematic representation of experimental groups. b Contusion (Controlled cortical impact model (CCI)) injury experimental design. c Schematic representation of the injury area, and image acquisition. d Representative images of Tuj1 neuronal staining at 24 h, 48 h, and 14d after injury in N, NA, NM, and NAM groups. e Quantification of Tuj1 neuronal network density in control and P110 treated groups of N, NA, NM, and NAM at three different time points (24, 48 h, and 14d). f Quantification of MAP2 protein Western blots at 1 h and 24 h after injury in all groups. Proteins were isolated from entire scaffolds without separating the injured area from the penumbra. Data presented in e and (f) mean ± SEM of three independent experiments with n = 3 for sham groups and n = 4 scaffolds for injury groups. *, **, *** indicate significant differences (p < 0.05, 0.01, 0.001 respectively; two-way ANOVA (analysis of variance) (with Tukey’s post-hoc test) between control and experimental groups). Scale bar: 50 µm.

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