Fig. 3: Injury-induced neurodegeneration in tricultures, but not neuronal monocultures, was associated with increased Drp1-Fis1 driven mitochondria fission. | Cell Death & Disease

Fig. 3: Injury-induced neurodegeneration in tricultures, but not neuronal monocultures, was associated with increased Drp1-Fis1 driven mitochondria fission.

From: Mitochondria dysregulation contributes to secondary neurodegeneration progression post-contusion injury in human 3D in vitro triculture brain tissue model

Fig. 3

a Representative images of TOMM20 mitochondria at 24 h after contusion in N, NA, NM, and NAM groups. b Frequency distribution (%) of TOMM20-positive mitochondria aspect ratio in control and P110 treated groups of N, NA, NM, and NAM. c TOMM20 protein quantification by Western blot 24 h after injury in all groups. d, e pDRP1/DRP1, and Fis1 protein quantification by Western blot 24 h after the injury in all groups. Proteins were isolated from entire scaffolds without separating the injured area from the penumbra. Data presented in (b-e) mean ± SEM of three independent experiments with n = 3 for sham groups and n = 4 scaffolds for injury groups. *, **, *** indicates significant difference (p < 0.05, 0.01, 0.001 respectively; two-way ANOVA (with Tukey’s post-hoc test) between control and experimental groups).

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