Fig. 1: ZIC2 was highly expressed in nasopharyngeal carcinoma. | Cell Death & Disease

Fig. 1: ZIC2 was highly expressed in nasopharyngeal carcinoma.

From: ZIC2 induces pro-tumor macrophage polarization in nasopharyngeal carcinoma by activating the JUNB/MCSF axis

Fig. 1: ZIC2 was highly expressed in nasopharyngeal carcinoma.

A Gene expression profiles of NPC cell lines, normal nasopharyngeal epithelial cell lines, and NPC tissues. (N01 is a normal nasopharyngeal epithelial cell. NPN_72 is nontumorous nasopharyngeal tissue. NPC_S and NPC_M is primarycultured NPC cells. NPC_66, NPC_62, NPC_64, NPC_49, NPC_46, NPC_54, NPC_52, NPC_41, NPC_51, NPC_60, NPC_53, NPC_57 are NPC tissues). B The ZIC2 expression in the GEO database. C RT-PCR (upper panel) and Western blotting (lower panel) were used to detect the expression of ZIC2 in immortalized nasopharyngeal epithelial cells and NPC cells. GAPDH served as a loading control for western blotting, and β-actin served as an internal control for RT-PCR. (N04 and N06 are normal nasopharyngeal epithelial cells. NP69, N5-tert, NPEC5-Bmi1, and NPEC2-Bmi1 are immortalized nasopharyngeal epithelial cells. CNE1, CNE2, HONE1, SUNE1, SUNE2, 5–8F, 6–10B, HNE1, HK1, S18, S26, and C666-1 are NPC cells). D The expression levels of ZIC2 after shRNA knockdown in the nasopharyngeal carcinoma cell lines (CNE2-EBV and HNE1-EBV) were detected by both RT-PCR and western blotting. β-actin served as an internal control for RT-PCR, and α-tubulin served as the internal loading control for western blotting. shctrl (negative control shRNA). E GSEA analysis of RNA sequencing data for the nasopharyngeal carcinoma cell lines CNE2-EBV (upper panel) and HNE1-EBV (lower panel) (shZIC2 vs. shctrl).

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