Fig. 4: ZIC2 promotes the M2 phenotype polarization of TAMs through the regulation of JUNB in NPC cells. | Cell Death & Disease

Fig. 4: ZIC2 promotes the M2 phenotype polarization of TAMs through the regulation of JUNB in NPC cells.

From: ZIC2 induces pro-tumor macrophage polarization in nasopharyngeal carcinoma by activating the JUNB/MCSF axis

Fig. 4: ZIC2 promotes the M2 phenotype polarization of TAMs through the regulation of JUNB in NPC cells.

A, B Representative image (left panel) and quantification (right panel) of the expression of the cytokines TNF-α, TGF-β, and related surface marker ARG-1 in macrophages cocultured with HK1-EBV cells overexpressing ZIC2 treated with JUNB-specific siRNA (A) or JUNB inhibitor, T5224 (B). NC (Negative control siRNA). C, D TNF-α, TGF-β, and IL-10 production was determined by ELISA analysis of the coculture supernatants of HK1-EBV cells overexpressing ZIC2 treated with (C) or JUNB inhibitor, T5224 (D). NC (Negative control siRNA). All of the experiments were repeated at least three times, and statistical analyses were conducted with an unpaired Student’s t-test; *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the corresponding control.

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