Fig. 3: circTBPL1 overexpression promotes cell proliferation and motility of breast cancer cells. | Cell Death & Disease

Fig. 3: circTBPL1 overexpression promotes cell proliferation and motility of breast cancer cells.

From: Cancer-associated fibroblasts facilitate breast cancer progression through exosomal circTBPL1-mediated intercellular communication

Fig. 3: circTBPL1 overexpression promotes cell proliferation and motility of breast cancer cells.The alt text for this image may have been generated using AI.

A The circTBPL1 overexpression efficiency and TBPL1 expression level were verified using qRT-PCR assay. B–D The proliferation of breast cancer cells after circTBPL1 overexpression was detected by MTT assay (B), colony formation assay (C), and Edu assay (D). E Transwell assay was performed to evaluate the effect of circTBPL1 overexpression on the migration and invasion abilities of breast cancer cells. F Wound healing assay was used to detect the cell migration after circTBPL1 overexpression in breast cancer cells. G Phalloidin staining was used to show the morphological change of breast cancer cells transfected with pLCDH-ciR or circTBPL1. H The expression of EMT-related markers was assessed by western blot. I Tube formation assay was performed to evaluate the angiogenesis-promoting effect of circTBPL1 overexpression in breast cancer cells. (ns, no significance, *P < 0.05, **P < 0.01, ***P < 0.001).

Back to article page