Fig. 8: Exosomal circTBPL1 derived from CAFs regulates miR-653-5p/TPBG axis in vivo to promote tumor growth and metastasis of breast cancer. | Cell Death & Disease

Fig. 8: Exosomal circTBPL1 derived from CAFs regulates miR-653-5p/TPBG axis in vivo to promote tumor growth and metastasis of breast cancer.

From: Cancer-associated fibroblasts facilitate breast cancer progression through exosomal circTBPL1-mediated intercellular communication

Fig. 8: Exosomal circTBPL1 derived from CAFs regulates miR-653-5p/TPBG axis in vivo to promote tumor growth and metastasis of breast cancer.The alternative text for this image may have been generated using AI.

A Nude mice were subcutaneously implanted with MDA-MB-231 cells co-injected with CAF pLCDH-ciR or CAF circTBPL1. The images of xenograft tumors in each group. B, C Tumor volume (B) and weight (C) were measured in each group. D, E qRT-PCR (D) and western blot (E) were used to detect the expression of related factors in tumor tissues. F Tumor tissues from xenograft model mice were subjected to HE staining, and IHC staining was performed to evaluate the expression of the related factors in tumor tissues. G Representative images of the lung tissues after tail vein injection of MDA-MB-231 cells with or without circTBPL1 overexpression. H Representative images of HE staining for lung tissues in each group. I The histogram analysis of the metastatic nodules number in per lung. J The schematic overview illustrates the mechanistic basis for the observational study results. CAF-derived circTBPL1 is transmitted into breast cancer cells through exosomes and can therein modulate the miR-653-5p/TPBG axis to influence tumor growth and metastasis. (**P < 0.01, ***P < 0.001).

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