Fig. 6: The phosphorylation status of G6PD was positively related to the immunoprecipitated SRC/GSTP1 in the ER/PR positive type or Her (-) type of breast cancer.
From: A GSTP1-mediated lactic acid signaling promotes tumorigenesis through the PPP oxidative branch
A Immunoprecipitated SRC/GSTP1 and Y249/Y322 phosphorylation status of G6PD that was immuno-enriched from clinical human breast cancer samples, as determined by Western-blots against the tumor (T) and adjacent normal tissues (N). B The G6PD Tyr phosphorylation status at 2 sites in (A) were quantified and statistically analyzed. Data are normalized to G6PD (IP) protein, presented as mean ± SD and analyzed by unpaired Students’ test, n = 13 pairs. P(Y249) = 0.0507, P(Y322) = 0.0009. C The immunoprecipitated GSTP1 and SRC expression levels in (A) were quantified and statistically analyzed. Data are normalized to G6PD (IP) protein, presented as mean ± SD and analyzed by unpaired Students’ test, n = 13 pairs. P(SRC) = 0.0009, P(GSTP1) = 0.0162. D A possible correlation between relative SRC/GSTP1 (IP) levels and relative p-Tyr levels of G6PD was determined in various types of breast cancer samples. Data are analyzed and shown by Pearson Correlation Coefficient. ER + /PR + : 5pairs; ER-/PR-: 4 pairs; ER-PR + : 3 pairs; HER-: 10 pairs.
