Fig. 3: ANAPC11 mediates FOXO3 degradation through the ubiquitin–proteasome pathway. | Cell Death & Disease

Fig. 3: ANAPC11 mediates FOXO3 degradation through the ubiquitin–proteasome pathway.

From: The APC/C E3 ligase subunit ANAPC11 mediates FOXO3 protein degradation to promote cell proliferation and lymph node metastasis in urothelial bladder cancer

Fig. 3: ANAPC11 mediates FOXO3 degradation through the ubiquitin–proteasome pathway.The alternative text for this image may have been generated using AI.

A Western blot analysis followed by IP in T24 and UM-UC-3 cells with IgG or anti-ANAPC11 antibody. B IP with anti-His or IgG in T24 and UM-UC-3 cells transfected with FOXO3-His or not. C Protein levels of ANAPC11, FOXO3, and GAPDH were measured by western blot analysis. D GST pulldown demonstrated by Coomassie blue staining (left) and western blotting (right). E Abundance of FOXO3 in sh-NC or sh-ANAPC11 UBC cells treatment with 30 μg/mL CHX, detected by western blotting. Band intensities were quantified by ImageJ and normalized to the intensity of 0 h. F Western blot analysis of cells overexpressing ANAPC11 treated with PBS or MG132 (20 μM). G Western blot analysis followed by IP evaluated the ubiquitination of FOXO3. Data are shown as mean ± SD. ANOVA, *P < 0.05, **P < 0.01.

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