Fig. 4: NUCKS1 elevates asparagine biosynthesis and alters sensitivity to L-asparaginase via ASNS. | Cell Death & Disease

Fig. 4: NUCKS1 elevates asparagine biosynthesis and alters sensitivity to L-asparaginase via ASNS.

From: NUCKS1, a LINC00629-upregulated gene, facilitated osteosarcoma progression and metastasis by elevating asparagine synthesis

Fig. 4: NUCKS1 elevates asparagine biosynthesis and alters sensitivity to L-asparaginase via ASNS.The alternative text for this image may have been generated using AI.

A Schematic diagram describing the reaction catalyzed by ASNS. B, C The relative enzyme activity of ASNS was assessed in MNNG/HOS and 143B cells with or without NUCKS1 knockdown. D The relative enzyme activity of ASNS was assessed in MNNG/HOS cells with or without NUCKS1 overexpression. E, F 0.01 mM aspartate (Asn) or glutamate (Glu) was added into NUCKS1-depleted cells. The proliferative abilities were measured with an EdU staining assay. Scale bar: 100 μm. G, H Intracellular amount of Asn was assessed in MNNG/HOS and 143B cells with or without NUCKS1 knockdown. I Intracellular amount of Asn was assessed in MNNG/HOS cells with or without NUCKS1 overexpression. J, K MNNG/HOS cells with or without NUCKS1 depletion were treated with 10 μM l-Asparaginase for 48 h. Cell death was measured by PI staining. Scale bar: 200 μm. Data in B–D, F–J were analyzed by Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001.

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