Fig. 9: LINC00629 activated NUCKS1/ASNS axis and promoted Asparagine synthesis through completely binding to miR-4768-3p. | Cell Death & Disease

Fig. 9: LINC00629 activated NUCKS1/ASNS axis and promoted Asparagine synthesis through completely binding to miR-4768-3p.

From: NUCKS1, a LINC00629-upregulated gene, facilitated osteosarcoma progression and metastasis by elevating asparagine synthesis

Fig. 9: LINC00629 activated NUCKS1/ASNS axis and promoted Asparagine synthesis through completely binding to miR-4768-3p.The alternative text for this image may have been generated using AI.

A miR-4768-3p was transfected into 143B and MNNG/HOS cells with or without LINC00629 overexpression. The expression levels of NUCKS1 and ASNS were detected by western blot. B The wild type (WT) of LINC00629 or the mutational binding site the miR-4768-3p (Mut) were transfected into 143B and MNNG/HOS cells. The expression levels of NUCKS1 and ASNS were detected by western blot. C, D The enzyme activity of ASNS and the intracellular Asn amount were assessed in MNNG/HOS cells with or without LINC00629 knockdown. E, F The WT or Mut of LINC00629 was transfected into MNNG/HOS cells. The enzyme activity of ASNS and the intracellular Asn amount were assessed. G–J The WT or Mut of LINC00629 was transfected into MNNG/HOS cells. Cell growth and migration were assessed by colony formation and Transwell assays. K–N miR-4768-3p was transfected into MNNG/HOS cells with or without LINC00629 overexpression. Cell growth and migration were assessed by colony formation and Transwell assays. Data in C–F, H, J, L and N were analyzed by Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001.

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