Fig. 4: Transcriptome analysis of LEF1 and KDM4A.

A Heatmap of coregulated gene expression after LEF1/KDM4A knockdown. Representative genes, GO terms, and KEGG pathways of upregulated or downregulated genes are also shown. LEF1 or KDM4A was knocked down in CAL-27 cells by using siRNAs. Three independent samples were separately subjected to RNA-seq analysis. B Box plot showing the fragments per kilobase of transcript per million mapped reads (FPKM) of gene expression distribution of upregulated or downregulated genes. For each sample, the target groups were separately compared to three control samples. C Pathway analysis of LEF1/KDM4A-regulated target genes arranged into functional groups. D Verification of RNA-seq results through qPCR analysis of the indicated genes in CAL-27 cells. Results are represented as fold-change compared to the control, with GAPDH used as the internal reference. E Heatmap of differentially expressed genes from the Hippo signalling pathway. F Verification of important genes in the Hippo signalling pathway through qPCR analysis in CAL-27 cells. Results are represented as fold-change compared to the control, with GAPDH used as the internal reference. Data represent mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.