Fig. 3: N-RAS deficiency aggravates BDL-induced liver injury and fibrosis. | Cell Death & Disease

Fig. 3: N-RAS deficiency aggravates BDL-induced liver injury and fibrosis.

From: Neuroblastoma RAS viral oncogene homolog (N-RAS) deficiency aggravates liver injury and fibrosis

Fig. 3: N-RAS deficiency aggravates BDL-induced liver injury and fibrosis.The alternative text for this image may have been generated using AI.

A Representative Sirius red (SR) staining (A) was performed in paraffin sections of N-RAS+/+ and N-RAS−/− livers, 28 days after CCl4. B Immunofluorescence for Collagen IA was carried out in the cryosections of the same animals. C Liver weight (LW) versus body weight (BW) ratio was calculated and represented. D Representative H&E staining of N-Ras+/+ and N-Ras−/− mice, 28 days after BDL. E Immunoblots were performed using pRIK1/3, pMLKL, CC3, CC8 antibodies. GAPDH was used as housekeeping protein. F Protein expression of α-SMA and PCNA was evaluated by Western blot. GAPDH was used as housekeeping protein. Values represent mean ± SEM from 5–6 mice per group (**p < 0.01; ***p < 0.001; ****p < 0.0001). Dotted areas indicate necrotic foci.

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