Fig. 4: NDUFS6 was the potential downstream effector of PRMT1 in MM. | Cell Death & Disease

Fig. 4: NDUFS6 was the potential downstream effector of PRMT1 in MM.

From: PRMT1 methylation of WTAP promotes multiple myeloma tumorigenesis by activating oxidative phosphorylation via m6A modification of NDUFS6

Fig. 4

A The density distribution of total m6A peaks in the NC and PRMT1 KD cells. B GSEA analysis of differentially expressed genes in PRMT1 KD cells based on m6A -seq. C Predominant consensus motif GGAC was detected in both NC and PRMT1 KD cells. D Integrative Genomics Viewer (IGV) tracks display m6A abundance in NDUFS6 transcripts in NC and PRMT1 KD cells. (E) MeRIP-qPCR analysis m6A enrichment of NDUFS6 transcripts in MM.1 S cells with or without si-PRMT1 72 h after transfection. F qRT-PCR and western blotting analysis NDUFS6 mRNA and protein level in MM.1 S cells transfected with si-NC or si-PRMT1, or EV and PRMT1-OE cells after 72 h. G Kaplan-Meier survival analysis of OS in GSE4581. NDUFS6 mRNA expression H and protein expression (I) in primary bone marrow mononuclear cells from NDMM and normal donors. J ROS level was assessed by flow cytometry in MM.1 S NC and NDUFS6-KD cells 72 h after the transfection. K Measurement of OCR transfected with si-NC and si-NDUFS6 in RPMI-8226 and MM.1 S cells after 72 h. Data represent the mean ± SD. Experiments were performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001. n.s. not significant.

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