Fig. 6: PRMT1 induced activation of oxidative phosphorylation through WTAP-NDUFS6 axis. | Cell Death & Disease

Fig. 6: PRMT1 induced activation of oxidative phosphorylation through WTAP-NDUFS6 axis.

From: PRMT1 methylation of WTAP promotes multiple myeloma tumorigenesis by activating oxidative phosphorylation via m6A modification of NDUFS6

Fig. 6

A ROS level was assessed by flow cytometry in RPMI-8226 cells transfected with EV+si-NC, EV+si-NDUFS6, PRMT1-OE+si-NC, and PRMT1-OE+si-NDUFS6 after 72 h. B ROS level was assessed by flow cytometry in MM.1 S cells transfected with si-NC, si-PRMT1, si-WTAP, and si-PRMT1+si-WTAP after 72 h. C Measurement of OCR in RPMI-8226 cells transfected with EV+si-NC, PRMT1-OE+si-NC, EV+si-NDUFS6, and PRMT1-OE+si-NDUFS6 after 72 h. D Measurement of OCR transfected with si-NC, si-PRMT1, si-WTAP, and si-PRMT1+ si-WTAP in MM.1 S after 72 h. E Western blotting analysis of NDUFS6 protein level in RPMI-8226 cells transfected with EV+si-NC, PRMT1-OE+si-NC, EV+si-NDUFS6, and PRMT1-OE+si-NDUFS6 after 72 h. F Western blotting analysis of NDUFS6 protein level in MM.1 S cells transfected with si-NC, si-PRMT1, si-WTAP, and si-PRMT1+ si-WTAP after 72 h. Data are expressed as the mean ± SD. Experiments were performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001. n.s. not significant.

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