Fig. 17: Determination of Heimdall biological function through experimental knockout using CRISPR-CAS9 technology. | Cell Death & Disease

Fig. 17: Determination of Heimdall biological function through experimental knockout using CRISPR-CAS9 technology.

From: Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome

Fig. 17

A–C Phenotype analyses of normal DI TNC1 astrocytes and Heimdall KO astrocytes revealed that Heimdall KO in astrocytes triggered the growth of extensions as revealed in time course from 1 day to 7 days. Scale bars: 50 μm. D Western blot analyses performed with anti-Heimdall, on protein cell extracts from control DI TNC1 astrocytes (CTRL) or Heimdall KO DI TNC1 astrocytes stimulated or not with 200 ng/mL of LPS (LPS) in reducing conditions. E Western blot analyses performed with anti-Heimdall, on secretome of control DI TNC1 astrocytes (CTRL) or DI TNC1 infected with empty vector (EV) or Heimdall KO DI TNC1 astrocytes stimulated or not with 200 ng/mL of LPS in non-reducing conditions. F Western blot analyses of GFAP and G its quantification after normalization with those of Actin. H Heimdall KO DI TNC1 astrocytes exhibit 4-fold higher depolarization state compared to control and EV condition (ANOVA p < 0.0001). I Heimdall KO DI TNC1 astrocytes exhibit 2-fold lesser rest membrane potential (mV) compared to control cells (ANOVA p < 0.0001).

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