Fig. 2: Transcriptomic and proteomic characterization of Heimdall. | Cell Death & Disease

Fig. 2: Transcriptomic and proteomic characterization of Heimdall.

From: Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome

Fig. 2

A Identification of Heimdall and its isoform by shot gun proteomic. Peptides covering Heimdall sequence are surrounded in blue rectangle for IP_1304331.1 and brown rectangle for IP_1304334.1. A sequence comparison with the variable kappa light chain is presented. B Amplification of Heimdall transcript. (a) Localization of the two primers used during RT-PCR experiment. (b) Amplification by RT-PCR of a fragment at 540 bp corresponding to Heimdall transcript was performed [1] on cDNA synthesized from DI TNC1 Astrocytes [2]. As a control to exclude genomic DNA contamination, the experiment was also carried out on a negative reverse transcriptase sample. (c) Alignment of the nucleic acid sequence encoding Heimdall identified by RT-PCR with the long non-coding RNA AABR07051592.2 retrieved from Ensembl database. (d) Alignment of the deduced amino acid sequence of Heimdall with the predicted sequence IP-1304343.1 encoded by the non-coding RNA AABR07051592.2.

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