Fig. 5: Key sites of TET2 were identified to be crucial for the interaction with β-catenin. | Cell Death & Disease

Fig. 5: Key sites of TET2 were identified to be crucial for the interaction with β-catenin.

From: Nuclear localization of TET2 requires β-catenin activation and correlates with favourable prognosis in colorectal cancer

Fig. 5: Key sites of TET2 were identified to be crucial for the interaction with β-catenin.The alt text for this image may have been generated using AI.

A Various TET2 deletion constructs were expressed in 293 T cells. IP assays were used to determine whether they interacted with β-catenin. B Various TET2 mutants were expressed in 293T cells. Tet2CD-mt failed to interact with β-catenin. C–D Tet2CD or Tet2CD-mt was overexpressed in SW480 and SW620 cells. The localization of the overexpressed proteins (C) and the abilities of the proteins to induce global DNA demethylation (D) were determined. E Overexpression of Tet2CD-mt did not affect the expression of the RORA-SPARC axis. All experiments were repeated at least 5 times (n ≥ 5), except the sequencing experiments. Additional statistical information is provided in Supplementary Table S8.

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