Fig. 5: NPM regulated Akt phosphorylation and Akt promoted liver fibrosis markers. | Cell Death & Disease

Fig. 5: NPM regulated Akt phosphorylation and Akt promoted liver fibrosis markers.

From: NPM promotes hepatotoxin-induced fibrosis by inhibiting ROS-induced apoptosis of hepatic stellate cells and upregulating lncMIAT-induced TGF-β2

Fig. 5

A Fibrogenic factor TGF-β1 up-regulated Akt phosphorylation in LX-2 cells. B NPM knockout or knockdown inhibited AKT phosphorylation in hepatic stellate cells LX-2. The knockout efficiency of NPM in LX-2 has been verified and shown in Fig. 2A. C The NPM inhibitor CIGB300 inhibited AKT phosphorylation in HSCs LX-2. D Akt phosphorylation inhibitor MK2206 reduced the protein expression of collagen I, MMP9, and α-SMA in LX-2 cells. E Akt activator SC79 up-regulated the expression of markers of liver fibrosis. F The collagen I and α-SMA were decreased or increased by Akt inhibitor or activator correspondingly in primary hepatic stellate cells. Data shown are representatives of three independent experiments. n ≥ 3; mean ± SEM; *p < 0.05, ***p < 0.001; T test.

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