Fig. 3: Ferrostatin-1 reverses the ability of SETD2 knockdown to increase sensitivity to erastin-induced ferroptosis.
From: Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC
A, B qRT‒PCR and western blot examined the efficiency of SETD2 knockdown transfected with the SETD2-specific shRNA. C CCK-8 assays were used to analyse the effect in the shCtrl, shSETD2, erastin, shS+E as well as shS+E+Fer-1 groups on cell viability in ACHN and the effect in the Ctrl, erastin as well as erastin+Fer-1 groups on cell viability in A498. D, E Flow cytometry was used to detect lipid peroxidation in the shCtrl, shSETD2, erastin, shS+E as well as shS+E+Fer-1 groups in ACHN and in the Ctrl, erastin as well as erastin+Fer-1 groups in A498. F, G Levels of MDA and Fe2+ were analyzed in the shCtrl, shSETD2, erastin, shS+E as well as shS+E+Fer-1 groups in ACHN and in the Ctrl, erastin as well as erastin+Fer-1 groups in A498. H GPX4, TF and TFR protein was detected in the shCtrl, shSETD2, erastin, shS+E as well as shS+E+Fer-1 groups in ACHN and in the Ctrl, erastin as well as erastin+Fer-1 groups in A498. I Electron microscopy imaging was conducted in the shCtrl, shSETD2, shS+E as well as shS+E+Fer-1 groups in ACHN, Black arrows marked mitochondrial changes. *P < 0.05, **P < 0.01, ***P < 0.001.
