Fig. 3: Depletion of Atg7 enhances Ref(2)P puncta formation and ployubiquitinated protein aggregates in Leon-depleted cells. | Cell Death & Disease

Fig. 3: Depletion of Atg7 enhances Ref(2)P puncta formation and ployubiquitinated protein aggregates in Leon-depleted cells.

From: The deubiquitinase Leon/USP5 interacts with Atg1/ULK1 and antagonizes autophagy

Fig. 3

A Confocal microscopy analysis of larval fat body expressing LucRNAi, LeonRNAi, Atg7RNAi or co-expressing LeonRNAi and Atg7RNAi with Cg-Gal4 driver and stained with Ub and Ref(2)P antibodies. B Quantification of the number and colocalization of Ref(2)P puncta and polyubiquitin aggregates in (A). The Pearson’s correlation coefficient was analyzed by imageJ. Scale bar, 10μm. Data as shown by mean ± SEM in N = 30 cells from three independent experiments, **P < 0.01; **** P < 0.0001; NS not significant.

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