Fig. 1: xCT is transiently upregulated in pancreatic dedifferentiating acinar cells.
From: Pancreatic acinar cell fate relies on system xC- to prevent ferroptosis during stress
a Schematic representation of our in vitro model of human and mouse exocrine acinar and duct cells where acinar cells dedifferentiate and acquire ductal characteristics. b Volcano plot of most differentially expressed genes comparing mouse dedifferentiating exocrine cells at day 1 (right, green dots) and exocrine cells at day 0 (left, purple dots). Key genes were in red dots with name labels. c Volcano plot of most differentially expressed genes comparing human dedifferentiated acinar cells (right, green dots) and duct cells (left, brown dots). d Protein levels of xCT assessed by western blot in human exocrine cell cultures with quantification (Mean ± SD; N = 4; unpaired t-test). e Protein levels of xCT assessed by western blot in WT and KO mouse exocrine cell cultures with quantification (Mean ± SD; N = 5; unpaired t-test). f Relative ROS level assessed by Fluorometric Intracellular ROS Kit in WT/KO mouse exocrine cells at day 0 (D0), day 1 (D1), and day 4 (D4), respectively. Intensity represented in square root of normalized fluorometric reads (Mean ± SD; N = 3; two-way ANOVA with multiple comparisons).
