Fig. 4: PHF23 promotes lung cancer progression through the activation of ERK signaling pathway. | Cell Death & Disease

Fig. 4: PHF23 promotes lung cancer progression through the activation of ERK signaling pathway.

From: PHF23 promotes NSCLC proliferation, metastasis, and chemoresistance via stabilization of ACTN4 and activation of the ERK pathway

Fig. 4: PHF23 promotes lung cancer progression through the activation of ERK signaling pathway.The alternative text for this image may have been generated using AI.

A GSEA in the TCGA database was performed. PHF23-related enrichment plots were shown. B Expression of proteins involved in the ERK signaling in A549 and H1299 cells treated with DMSO or the ERK inhibitor PD98059. C DNA replication of H1299 and A549 cells was determined by EDU staining with DMSO or the ERK inhibitor PD98059. Scale bar: 200 μm. Mean ± SD, n = 3. *P < 0.05; **P < 0.01; ***P < 0.001. D Viability of H1299 and A549 cells was analyzed by CCK8 24 h after treatment with the ERK inhibitor PD98059 and different concentrations of cisplatin or docetaxel. E γ-H2AX foci formation in H1299 cells with the ERK inhibitor PD98059 was detected by Immunofluorescence 24 h after treatment with cisplatin (15 μmol/L) or docetaxel (25 μmol/L). Mean ± SD, n = 3. ***P < 0.001.

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