Fig. 3: Circ_0042881 serves as a miR-217 sponge. | Cell Death & Disease

Fig. 3: Circ_0042881 serves as a miR-217 sponge.

From: EIF4A3-mediated circ_0042881 activates the RAS pathway via miR-217/SOS1 axis to facilitate breast cancer progression

Fig. 3: Circ_0042881 serves as a miR-217 sponge.

A, B RIP experiments were performed, and RT‐qPCR assays and agarose gel electrophoresis were used to detect the enrichment of circ_0042881 to AGO2. C Venn diagram representing the potential targeted miRNAs of circ_0042881 by CircInteractome, circBANK, Starbase v3.0. D RT-qPCR analyzed the relative expression of 4 candidate miRNAs in MDA-MB-231 and MCF-7 after transfecting with si-circ_0042881. E The relative expression of miR-217 and miR-338-5p was tested by RT-qPCR after circ_0042881 overexpression. F To examine the direct interaction between circ_0042881 and miR-217, the luciferase reporter vectors containing circ_0042881 WT or circ_0042881 MUT were constructed. G The correlation between circ_0042881 and miR-217 in BC tissues was analyzed by Pearson correlation analysis. H The luciferase activities in MDA-MB-231 and MCF-7 cells co-transfected with miR-217 mimic or mimic NC and luciferase reporters containing circ_0042881 WT or circ_0042881 MUT. The data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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