Fig. 7: CRTAC1 increases cisplatin-induced intracellular calcium levels by releasing calcium through the RyR channel. | Cell Death & Disease

Fig. 7: CRTAC1 increases cisplatin-induced intracellular calcium levels by releasing calcium through the RyR channel.

From: CRTAC1 enhances the chemosensitivity of non-small cell lung cancer to cisplatin by eliciting RyR-mediated calcium release and inhibiting Akt1 expression

Fig. 7: CRTAC1 increases cisplatin-induced intracellular calcium levels by releasing calcium through the RyR channel.The alternative text for this image may have been generated using AI.

A, C Calbryte 630 staining was used to detect the intracellular calcium level in H1299/HCC827 (CRTAC1/vector) cells pretreated with 2.5 μM BAPTA for 30 min and 20 μM CDDP for 48 h. B, D The relative calcium level was calculated in H1299/HCC827 (CRTAC1/vector) cells. E, F H1299/HCC827 (CRTAC1/vector) cells were pretreated with 2.5 μM BAPTA for 30 min and 20 μM CDDP for 48 h, and STUB1 expression was detected by western blotting. G, H H1299/HCC827 (CRTAC1/vector) cells were pretreated with BAPTA (2.5 μM) for 30 min and 20 μM CDDP for 48 h, and STUB1 mRNA expression was detected by qRT-PCR. I, J The activity of the STUB1 promoter was detected using a dual-luciferase reporter assay in cells treated with BAPTA and cisplatin. K, M H1299/HCC827 (CRTAC1/vector) cells were pretreated with BAPTA and treated with cisplatin, and apoptosis was detected by flow cytometry. L, N Statistical analysis of apoptosis in H1299/HCC827 (CRTAC1/vector) cells treated with BAPTA and cisplatin. O–R H1299/HCC827 (CRTAC1/vector) cells were pretreated with dantrolene (25 μM) for 2 h and incubated with cisplatin (20 μM) for 48 h. The relative calcium level was measured by flow cytometry. S–V H1299/HCC827 (CRTAC1/vector) cells were pretreated with dantrolene (25 μM) for 2 h and treated with cisplatin (20 μM) for 48 h, and apoptosis was detected by flow cytometry. (*) indicates a significant difference (P < 0.05).

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