Fig. 3: GSDMD deficiency inhibited psoriasis-like inflammation in mice stimulated by imiquimod or IL-23.
From: Gasdermin D-mediated keratinocyte pyroptosis as a key step in psoriasis pathogenesis
A–F WT (C57BL/6NGpt) and Gsdmd−/− mice were stimulated by imiquimod or vaseline, respectively. A Skin appearances were presented. B The severity of the lesions was evaluated by PASI scores (for each group, n = 6). C The epidermal thickness was measured by Image J software (for each group, n = 6). D Histological features were analyzed by H&E staining. Immunohistochemistry study showed the levels of Ki-67 (n = 6). E The levels of loricrin, K5 and MPO were detected by immunohistochemistry study (n = 6). F Western blotting assay was used to detect the level of cleaved GSDMD and cleaved IL-1β in epidermis lysate of WT or Gsdmd−/− mice (n = 3). G WT and Gsdmd−/− mice were intradermally injected with recombinant mouse IL-23 (1 μg) in the right ear. The left ear was intradermally injected with vehicle. Skin appearances after injection were presented. H The severity of the lesions was evaluated by PASI scores (for each group, n = 4). I The epidermal thickness was measured by Image J software (for each group, n = 4). J Histological features were analyzed by H&E staining. Scale bar represents 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001. GSDMD-FL GSDMD full length, N-GSDMD N-terminal GSDMD.
