Fig. 5: MiR-297 targets and down-regulates PTBP3 to inhibit the PI3K/AKT signaling pathway.
From: MiR-297 inhibits tumour progression of liver cancer by targeting PTBP3
A The candidate target genes of miR-297 was predicted by four different prediction algorithms: Targetscan, PicTar, miRWalk and miRDB. B The predicted binding sites of miR-297 in the 3’ UTR of PTBP3 and corresponding mutant sites of PTBP3. C RT-qPCR detection of the expression of PTBP3, PI3K/AKT signaling pathway-related factors and EMT related markers in HepG2 and Huh7 cells transfected with hsa-miR-297 mimic or PTBP3 plasmid. D Western blot analysis of protein expression of PTBP3, PI3K/AKT signaling pathway-related factors and EMT related markers in HepG2 cells and Huh7 cells transfected with hsa-miR-297 mimic or PTBP3 plasmid. *p < 0.05 vs. the NC treatment. #p < 0.05 vs. HCC cells transfected with hsa-miR-297 mimic. Cell experiments were repeated for three times. E, F The Luciferase reporter assays was conducted at 48 h after co-transfection of HepG2 cells with the PTBP3 WT/PTBP3 MUT plasmid and hsa-miR-297 mimics or inhibitor. *P < 0.05, **P < 0.01 and ***P < 0.001.
