Fig. 4: SPDEF was involved in cancer stem cell-like characteristics and tumorigenesis by directly regulating GALNT7 expression in luminal BC. | Cell Death & Disease

Fig. 4: SPDEF was involved in cancer stem cell-like characteristics and tumorigenesis by directly regulating GALNT7 expression in luminal BC.

From: SPDEF enhances cancer stem cell-like properties and tumorigenesis through directly promoting GALNT7 transcription in luminal breast cancer

Fig. 4: SPDEF was involved in cancer stem cell-like characteristics and tumorigenesis by directly regulating GALNT7 expression in luminal BC.The alternative text for this image may have been generated using AI.

A Pearson test was performed to determine the correlation between SPDEF and six candidate genes in luminal BC. B The expression level of six candidate genes in luminal BC compared to non-cancerous samples by TCGA database. C GALNT7 abundance in sh-NC and sh-SPDEF of MCF7 and T47D cells measured by RT-qPCR. D Protein abundance of GALNT7 in sh-NC and sh-SPDEF measured by Western-blotting. E GALNT7 abundance in shNC+OE-Vector, sh-SPDEF + OE-vector and sh-SPDEF + OE-GALNT7 cells measured by RT-qPCR. sh-NC was the negative control sh-RNA, sh-SPDEF was the knockdown of SPDEF, OE-vector was the negative control for the gene over-expression, OE-GALNT7 was the over-expression of GALNT7. F Protein abundance of GALNT7 in shNC+OE-Vector, sh-SPDEF + OE-vector and sh-SPDEF + OE-GALNT7 cells measured by Western-blotting. G Proliferation activities of shNC+OE-Vector, sh-SPDEF + OE-vector and sh-SPDEF + OE-GALNT7 measured by the CCK-8 assay. H-K. Representative images of migrated and invaded cells and quantification assay of shNC+OE-Vector, sh-SPDEF + OE-vector and sh-SPDEF + OE-GALNT7 in MCF7 (H–I) and T47D cells (J–K), scale bar = 60 μm. L–M Scratch wound healing assay results (L) and quantitative analysis (M). Images of all groups at 0 h and 48 h time intervals post injury, scale bar = 120 μm. N. Expression of stem genes in shNC+OE-Vector, sh-SPDEF + OE-vector and sh-SPDEF + OE-GALNT7 of MCF7 and T47D cells by RT-qPCR. O-P. The morphology of spheroid formation (O) and quantitative analysis (P) in shNC+OE-Vector, sh-SPDEF + OE-vector and sh-SPDEF + OE-GALNT7 of MCF7 and T47D cells, scale bar = 120 μm. Q-R. The soft agar colony formation assay (Q) and quantitative analysis (R) in shNC+OE-Vector, sh-SPDEF + OE-vector and sh-SPDEF + OE-GALNT7 of MCF7 and T47D cells. S-U. Subcutaneous tumors were harvested after sh-SPDEF cells infected with OE-GALNT7 in BALB/c nude mice. S tumor volume, T tumor growth curve, U tumor weight. *P < 0.05, **P < 0.01, ***P < 0.001.

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