Table 2 Antibodies used for validating the results.

From: Decoding the transcriptome of Duchenne muscular dystrophy to the single nuclei level reveals clinical-genetic correlations

Target

Clone

Host

Work dilution

Cell target

TE-7

TE-7

Mouse

1/80

Fibroblast

Collagen I

Polyclonal

Mouse

1/50

Extracellular matrix

Collagen VI

EPR17072

Rabbit

1/30

Extracellular matrix

Collagen III

EPR17673

Rabbit

1/15

Extracellular matrix

Laminin

4H8-2

Rat

1/5

Muscle fiber

CD31

PECAM1

Mouse

1/50

Endothelial cell

PDGFR alpha

Polyclonal

Goat

1/15

FAPs

CTGF

Polyclonal

Rabbit

1/10

Profibrotic cytokine

PDGF-AA

Polyclonal

Rabbit

1/10

Profibrotic cytokine

CD56

NCAM16.2

Mouse

1/50

Satellite cells / Regenerative fibers

MYH3

Polyclonal

Rabbit

1/100

Regenerative fibers

CD206

15-2

Mouse

1/50

M2 macrophages

TNNT2

1C11

Mouse

1/50

Regenerative fibers

ITGB1

12G10

Mouse

1/50

Muscle fiber

  1. PDGFR platelet derived growth factor, CTGF connective tissue growth factor, MYH3 Myosin heavy chain 3, TNNT2 Troponin T2, ITGB1 Integrin subunit beta 1.