Fig. 7: Blocking eIF5A^hpu reduced tumor infiltration of M2-like TAMs in syngeneic orthotopic murine tongue SCC tissues.

A–D Blocking eIF5A^hpu by GC-7 treatment reduced the infiltration of total CD11b⁺ monocytes and CD11b⁺/CD206⁺ M2-like macrophages in murine OSCC tissues as determined by flow cytometric analysis, whereby the representative histograms of FCM (A, C) and the mean percentage of CD11b⁺ and CD11b⁺CD206⁺ cells (n = 3) were presented, respectively (B, D). E representative immunofluorescence staining for F4/80 (green) and CD206 (red) in murine OSCC tissues from GC-7-treated or non-treated mice. The nuclei were counterstained with DAPI. Scale bar = 20 μm. F Semiquantification of fluorescence intensity (FI) of F4/80 and CD206 as well as the IF ratio of CD206⁺ versus F4/80⁺ in murine OSCC tissues from GC-7-treated or non-treated mice. G Representative IHC staining for arginase-1 (Arg1) protein expression in murine OSCC tissues from GC-7 treated or non-treated mice. Scale bars, 200 μm (the first panel), 100 μm (the second panel), 50 μm (the third panel), 20 μm (the fourth panel). The blue arrows indicate the partially enlarged views of the boxed areas. The red arrows point to the intrusion/invasion of ARG1⁺ M2-like TAMs in mice tongue tissues. H Semiquantitative analysis of the IHC staining intensity using ImageJ software.*P < 0.05; **P < 0.01; ***P < 0.001, GC-7 treatment versus non-treatment (mean ± SD).