Fig. 5: BCL2L13 overexpression promotes mitophagy in GBM cells in vitro.
From: BCL2L13 promotes mitophagy through DNM1L-mediated mitochondrial fission in glioblastoma
A Overexpression of BCL2L13 in A172 cells (A172-BCL2L13) was confirmed by western blot. B, C Western blotting analysis was performed to detect levels of LC3B in A172-EV/BCL2L13 cells after exposure to 3-MA (10 mM) or Baf (100 nM) for 48 h. D Confocal microscopy images of autophagy inhibitors (3-MA or Baf) pretreated A172-EV/BCL2L13 cells after co-expressing RFP-mito and GFP-LC3B (scale bars: 10 μm). E Cell viability of A172-BCL2L13 cells after autophagy inhibitors (3-MA or Baf) incubation. F Representative images and statistic results of migration and invasion ability of A172-EV/BCL2L13 cells after autophagy inhibitors (3-MA or Baf) incubation. All data are expressed as the mean ± SD of values from experiments performed in triplicate. *P < 0.05, **P < 0.01, and ***P < 0.001 compared between the two treatments indicated.
