Fig. 5: AURKAIP1 stabilized DDX5 by blocking ubiquitin-degradation of DDX5.
From: AURKAIP1 actuates tumor progression through stabilizing DDX5 in triple negative breast cancer
A TNBC cells transfected with scramble or AURKAIP1 siRNAs were treated with cycloheximide (CHX) for indicated time periods and protein stability of DDX5 was measured by WB. B The protein expression of DDX5 was detected by western blots in TNBC cells transfected with scramble or AURKAIP1 siRNAs for 48 h and treated with proteasome inhibitor MG132 (50 μM) for 6 h. C AURKAIP1 reduced DDX5 ubiquitination. The HEK 293T cells were transfected with HA-DDX5, MYC-Ub and Flag-AURKAUP1 or shAURKAIP1 plasmids and treated with 25 μM MG132 for 6 h. Immunoprecipitation and immunoblotting were performed using the selected antibodies.
