Fig. 6: AURKAIP1 activated Wnt/β-catenin signaling pathway through targeting DDX5.
From: AURKAIP1 actuates tumor progression through stabilizing DDX5 in triple negative breast cancer
A Veen diagram was used to present the intersection results of Gene Set Enrichment Analysis (GSEA) in different GEO datasets. B Representative plots of GSEA results. C Scatter plot showing the expression correlation between AURKAIP1 and Wnt/β-catenin signaling scores. D Assays of TOP/FOP luciferase activity was conducted to examine the Wnt activity in the HEK 293T cells transfecting with scramble or AURKAIP1 siRNAs. E Western blot analysis of β-catenin, CyclinD1, c-Myc and Met in indicated cells. F Immunofluorescence analysis of β-catenin in MDA-MB-231 cells transfected with scramble or AURKAIP1 siRNAs. The β-catenin rescued CCK8 (G) and transwell migration (H) assays revealed that AURKAIP1 promoted TNBC proliferation and migration via Wnt/β-catenin signaling pathway. I TOP/FOP flash reporter assay in indicated HEK 293T cells was performed to reaffirm that DDX5 overexpression could reverse the reduction of Wnt/β-catenin transcriptional activity induced by AURKAIP1 knockdown. J Western blot was used to detect the protein levels of β-catenin, CyclinD1, c-Myc and Met in indicated cells to verify whether DDX5 overexpression could invert the effect of AURKAIP1 silencing on essential proteins of Wnt/β-catenin signaling pathway. RT-qPCR (K) and western blots (L) analyses were performed to investigate the upstream-downstream relationship between AURKAIP1, DDX5 and β-catenin in the indicated cells.
