Fig. 5: METTL16 and MAT2A regulate intracellular GSH and ROS levels. | Cell Death & Disease

Fig. 5: METTL16 and MAT2A regulate intracellular GSH and ROS levels.

From: METTL16 controls Kaposi’s sarcoma-associated herpesvirus replication by regulating S-adenosylmethionine cycle

Fig. 5: METTL16 and MAT2A regulate intracellular GSH and ROS levels.

A iSLK-RGB-BAC16 cells transfected with siRNAs targeting MAT2A (siMA-1 and siMA-2) or the control siRNA (siCtrl) were treated with 3 mM of NaB for 24 h. The intracellular GSH level was analyzed by mass spectrometry. B iSLK-RGB-BAC16 cells transfected with siRNAs targeting METTL16 (siM16-1 and siM16-2) or the control siRNA (siCtrl) were treated with 3 mM of NaB for 24 h. The intracellular GSH level was analyzed by mass spectrometry. C iSLK-RGB-BAC16 cells transfected with siRNAs targeting MAT2A or the control siRNA were treated with 3 mM of NaB. The intracellular ROS level was measured and quantified by flow cytometry. D iSLK-RGB-BAC16 cells transfected with siRNAs targeting METTL16 or the control siRNA were treated with 3 mM of NaB. The intracellular ROS level was measured and quantified by flow cytometry. E ROS levels in iSLK-RGB-BAC16 cells treated with 3 mM of NaB and cycloleucine (Cyclo) at the indicated concentrations were measured by flow cytometry. F Intracellular ROS levels of iSLK-RGB-BAC16 cells treated with 3 mM of NaB and SAM at the indicated concentrations were measured by flow cytometry. *, **, and *** indicate p values of <0.05, <0.01, and <0.001, respectively, ns not significant.

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