Fig. 2: FBXW2 inhibits breast cancer progression through Moesin.
From: FBXW2 suppresses breast tumorigenesis by targeting AKT-Moesin-SKP2 axis
A Trypan blue exclusion assay in MCF7 cells expressing either NS or shRNAs against Moesin or FBXW2 or both. B Long-term colony formation assay in MCF7 cells expressing either NS or shRNAs against Moesin or FBXW2 or both. C Quantification of colonies from (B). D Anchorage-independent soft agar assay in MCF7 cells expressing either NS or shRNAs against Moesin or FBXW2 or both. Scale bars = 8 µM. E Quantification of colonies from (D). F Invasion assay in MCF7 cells expressing either NS or shRNAs against Moesin or FBXW2 or both. Scale bars = 100 µM. G Quantification of invaded cells from (F). H Scratch wound healing assay in MCF7 cells expressing either NS or shRNAs against Moesin or FBXW2 or both. Scale bars = 100 µM. I Quantification of migrated cells from (H). J Xenograft assay showing tumors of 4T1 cells expressing either NS or shRNAs against Moesin or FBXW2 or both. Cells were orthotopically injected in mammary fat pad of NOD-SCID female mice (4–6 weeks old). K Xenograft assay shows the progression of tumor growth of 4T1 cells expressing either NS or shRNAs against Moesin or FBXW2 or both in NOD-SCID female mice. L Representative IHC staining of FBXW2 and Moesin in tumors derived from NOD-SCID mice. 4T1 cells expressing either NS or shRNAs against Moesin or FBXW2 or both were injected orthotopically. Scale bars = 40 µM.
