Table 1 Antibody list.

IGF-I Receptor β

Western blotting /immunostaining

1:1000/1:50

Cell signaling, Danvers, MT, USA

Phospho-IGF-I Receptor β (Tyr1135/1136)

Western blotting

1:1000

Cell signaling

Human/mouse IGF-I Receptor Antibody (Mouse IgG1)

Functional Blocking

20 µg/mice

R&D systems, Minneapolis, MN, USA

Mouse IgG1 Antibody

Control of functional Blocking

20 µg/mice

R&D systems

IGF-I Antibody

Western blotting

1:1000

Abcam, Cambridge, UK

IGF-II Antibody

Western blotting

1:1000

Abcam

Phospho-mTOR (Ser2448) Antibody

Western blotting

1:1000

Cell signaling

Phospho-mTOR (Ser2481) Antibody

Western blotting

1:1000

Cell signaling

mTOR (7C10) Rabbit Monoclonal Antibody

Western blotting

1:1000

Cell signaling

Phospho-FoxO1 (Ser256) Antibody

Western blotting

1:1000

Cell signaling

FoxO1 (C29H4) Rabbit Monoclonal Antibody

Western blotting

1:1000

Cell signaling

Phospho-Akt (Thr308) Antibody

Western blotting

1:1000

Cell signaling

Akt Antibody

Western blotting

1:1000

Cell signaling

Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit Monoclonal Antibody

Western blotting

1:1000

Cell signaling

p70 S6 Kinase (49D7) Rabbit Monoclonal Antibody

Western blotting

1:1000

Cell signaling

LC3B Antibody

Western blotting

1:1000

Cell signaling

GAPDH Antibody (5A12)

Western blotting

1:10,000

Fujifilm Wako Pure Chemical Co, Osaka, Japan

PAX7 Antibody

Immunostaining

1:50

Developmental Studies Hybridoma Bank(DSHB), Iowa City, IA, USA

Laminin-2 (α-2 Chain) Antibody (4H8-2)

Immunostaining

1:1500

Sigma-Aldrich Japan/Merck, Tokyo, Japan

MyoD Antibody(G-1)

Immunostaining

1:50

Santa Cruz Biotechnology, Dallas, TX, USA

Ki67 Antibody

Immunostaining

1:500

Abcam

Donkey anti-mouse IgG F(ab’)2 (H + L) Alexa Fluor 488

Immunostaining

1:1000

Jackson immunoResearch Laboratories, West Grove, PA, USA

Donkey anti-Rabbit IgG (H + L) Alexa Fluor 647

Immunostaining

1:1000

Jackson immunoResearch Laboratories

Donkey anti-Rat IgG (H + L) Cy3

Immunostaining

1:1000

Jackson immunoResearch Laboratories

Donkey anti-Goat IgG (H + L) Alexa Fluor 647 plus

Immunostaining

1:1000

Molecular probes/Thermo Fisher Scientific, Waltham, MA, USA

Goat anti-mouse IgG (H + L) HRP

Immunostaining

1:5000

Jackson immunoResearch Laboratories

Goat anti-Rabbit IgG (H + L) HRP

Immunostaining

1:5000

Jackson immunoResearch Laboratories

α-Bungarotoxin-Alexa Fluor 594

Immunostaining

1:400

Molecular probes/Thermo Fisher Scientific

Donkey anti-mouse IgG (H + L)

Blocking

1:33 (3%)

Jackson immunoResearch Laboratories