Fig. 2: Phenotypic characterization of mice tumoroids by immunohistochemistry and immunofluorescence. | Cell Death & Disease

Fig. 2: Phenotypic characterization of mice tumoroids by immunohistochemistry and immunofluorescence.

From: A new scaffold-free tumoroid model provides a robust preclinical tool to investigate invasion and drug response in Renal Cell Carcinoma

Fig. 2

A 786-O and ACHN tumoroids and their counterpart tissue stained with Hematoxylin and Eosin (HE). Images were taken with AxioVision Zeiss microscope. Scale bar = 100 µm. B–E Immunofluorescence images of ACHN tumoroids and tissue that were taken using the LSM 880 AiryScan confocal microscope (Zeiss). Scale bar = 50 µm. All samples are stained with Hoechst. B Tumoroids labeled with CAIX, Ki67, Vimentin and FAP. C Tumoroids and their corresponding tissue labeled with Collagen Pan and CD44 (a–c), CAIX and Ki67 (d–f) and CD68 and actin (g–i). D Tumoroids labeled with CD31 and E-cadherin at different time point (7, 12 and 15 days) and the corresponding tissue. E Tumoroids labeled with FAP and vimentin (a), and Collagen Pan and CD44 (b).

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