Fig. 4: hnRNPA2B1 is required for CRNDE regulation of CRC cell proliferation and metastasis.

The hnRNPA2B1 overexpression plasmid or its empty vector was transfected into stable knockdown CRNDE CRC cells SW480 and DLD1 and their proliferative (A), invasive and migratory (B–D) abilities were assessed by functional rescue assays. n = 3 independent biological replicates. Scale bar, 100 μm (B); 200 μm (D). Functional rescue assays were performed in CRNDE overexpression cells with si-hnRNPA2B1 or si-NC transfection to examine the alterations in cell proliferation (E) and metastatic (F–H) capacity. n = 3 independent biological replicates. Scale bar, 100 μm (F); 200 μm (H). A two-tailed unpaired Student’s t-test and one-way ANOVA were used for statistical analysis, respectively. *P < 0.05, **P < 0.01, ***P < 0.001. Data represent mean ± SD.