Fig. 7: PRMT6-mediated tumor metastasis relied on STAT3 R729 methylation.
From: PRMT6 methylation of STAT3 regulates tumor metastasis in breast cancer
A Immunoblotting assay confirming the successful construction of cancer cells stably expressing STAT3 WT+ vector, STAT3 WT + PRMT6, STAT3 R729K+ vector, and STAT3 R729K + PRMT6. B Immunoblotting assays to detect the expression level of targeting proteins in cancer cells stably expressing STAT3 WT+ vector, STAT3 WT + PRMT6, STAT3 R729K+ vector, and STAT3 R729K + PRMT6. C RT-PCR assays to detect the mRNA expression level of markers of epithelial–mesenchymal transition of cancer cell stably expressing STAT3 WT+ vector, STAT3 WT + PRMT6, STAT3 R729K+ vector, and STAT3 R729K + PRMT6; n = 3, P < 0.05. D Statistical analysis for trans-well assays in Supplementary Fig. 6A; n = 3, P < 0.05. E Representative image of mouse lung metastasis experiments. F Statistical analysis of lung nodules in D; n = 5, P < 0.05. G Statistical analysis of the weight of the mice lungs in D; n = 5, P < 0.05. H Kaplan–Meier Plot was drawn in four groups of mouse lung metastasis assays; n = 10, P < 0.05. All immunoblotting assays were conducted three times, and the results were the same.
