Fig. 3: Repin1 knockdown mitigated iron-overload-induced suppression of osteogenesis and mineralization.
From: REPIN1 regulates iron metabolism and osteoblast apoptosis in osteoporosis
A Representative images of ARS staining at 21 days. B Representative images of ALP staining at 7 days. C Relative ALP expression level (fold change). D Semiquantitative evaluation of the ARS recovery ratio (fold change). Cells after lentivirus transfection were exposed to FAC at various concentrations (0–200 μmol/L) during osteogenic induction. E The expression levels of RUNX2, OSX, COL1A1 and ALP in MC 3T3 E1 cells were analyzed by western blotting after 4 d of osteogenic induction in the presence of 200 μmol/L FAC. F–I Quantitative analysis of western blotting. J–M The mRNA levels of Runx2, Osx, Col1a1 and Alp were analyzed. (Values are shown as the means ± SDs. *p < 0.05, **p < 0.01 and ***p < 0.005, n = 3 per group, all studies were performed with at least three biological replicates).
