Fig. 7: Repin1 knockdown prevents iron-overload-induced bone loss in vivo.
From: REPIN1 regulates iron metabolism and osteoblast apoptosis in osteoporosis
A Schematic diagram showing the in vivo experimental design. B, C Representative micro-CT reconstruction images of trabecular bone under the distal femur growth plate in the control group, NC + FAC group, FAC group, and sh-Repin1 + FAC group are shown. D Representative H&E staining images of trabecular bone under the distal femur growth plate from the control group, NC + FAC group, FAC group, and Sh-repin1+FAC group are shown. E Alizarin red double-label experiment. F Quantitative analysis of the width of the alizarin red double label. G–M Quantitative analysis of bone parameters. The region of interest selected for trabecular analysis started 100 sections below the proximal end of the distal femur growth plate, and 150 slices (6 µm each) were read per sample. G BMD (g/cm3). H BV (mm3). I BV/TV (%). J BS/BV (1/mm). K Tb. Th (mm). L Tb. N (1/mm). M Tb.sp (mm). (Values are shown as the means ± SDs. *p < 0.05, **p < 0.01 and ***p < 0.005, n = 6 biologically independent mice per group).
