Fig. 2: Cell viability evaluation and live cell cycle detection. | Cell Death & Disease

Fig. 2: Cell viability evaluation and live cell cycle detection.

From: The oleic/palmitic acid imbalance in exosomes isolated from NAFLD patients induces necroptosis of liver cells via the elongase-6/RIP-1 pathway

Fig. 2: Cell viability evaluation and live cell cycle detection.

Cell viability was evaluated by MTS assay, on Hepa-RG cells, after incubation with Healthy-Exo and NAFLD-Exo samples (A, B), alone or in combination with free PA (fPA) or OA (fOA) at different concentrations (5–100 µM), hybrid exosomes (PA/Healthy-Exo and OA/NAFLD-Exo) and fPA or fOA at 100 μM. Representative bright-field images of live Hepa-RG cells stained with CellClock™ Dye Reagent to monitor the four major cell cycle phases: dark green-blue (G2/M phases), light green (S phase) and Yellow (G0/G1 Phases) (C). Cells were treated with exosomes derived from healthy subjects and NAFLD patients, or hybrid exosomes loaded with FA. Scale bar 50 μm. The histogram represents the quantification of differences (%) between the different cell cycle phases (D). The exosomes concentration in terms of the total protein content was fixed at 20 µg/µL. The PA and OA concentrations were 10 and 13.5 µM respectively in PA/Healthy-Exo and OA/NAFLD-Exo samples. Negative controls were untreated cells (CTR). Experiments were repeated three times. **P < 0.001.

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