Fig. 5: Intracellular expression of RIP-1, phospho-RIP-1, GSDMD, and TGF-β1 in Hepa-RG cells. | Cell Death & Disease

Fig. 5: Intracellular expression of RIP-1, phospho-RIP-1, GSDMD, and TGF-β1 in Hepa-RG cells.

From: The oleic/palmitic acid imbalance in exosomes isolated from NAFLD patients induces necroptosis of liver cells via the elongase-6/RIP-1 pathway

Fig. 5: Intracellular expression of RIP-1, phospho-RIP-1, GSDMD, and TGF-β1 in Hepa-RG cells.

Representative western blotting of Hepa-RG cells treated with pristine exosomes (A) and hybrid exosomes (B) for RIP-1, phospho-RIP-1, GSDMD and TGF-β1, normalized using AKT and LAMIN A/C housekeeping proteins for the cytoplasm (C) and nuclei (N), respectively. Semi-quantitative evaluation, performed by means of video-densitometry, of the relative expression levels of RIP-1, phospho-RIP-1, GSDMD, and TGF-β1, normalized using AKT and LAMIN A/C for the cytoplasm, and nuclei, respectively, in the cells treated with exosomes and hybrid exosomes, C and D, respectively. Negative controls are untreated cells. *P < 0.001 and **P < 0.005. For all the experiments, the exosomes concentration was fixed at 20 µg/µL in terms of total protein content. The PA and OA concentration was 10 and 13.5 µM in PA/Healthy-Exo and OA/NAFLD-Exo samples, respectively.

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