Fig. 7: ELOVL6, phospho-RIP-1, and phospho-MLKL expression in Hepa-RG cells.
A Representative western blotting of ELOVL6, phospho-RIP-1, and phospho-MLKL (upper panel) with the corresponding densitometry analysis of protein level (lower panel) in silenced Hepa-RG cells, after incubation with pristine exosomes only or in combination with transfection complex; 20 μg of total protein extract were loaded. Negative control (CTR) was untreated cells and the vehicle was cells with si‐PORT‐NeoFX transfection agent only; GAPDH was used to normalize the targeted protein for each sample considered. **P < 0.001 vs negative control. B Representative western blotting of RIP-1, phospho-RIP-1 and phospho-MLKL (upper panel) with the corresponding densitometry analysis of protein level (lower panel), upon incubation of Hepa-RG cells with exosomes, only or in combination with Necrostatin-1 (NEC); 20 μg of total protein extract were loaded. Negative control (CTR) was untreated cells and GAPDH was used to normalize the targeted protein for each sample considered. **P < 0.001 vs negative control. C ROS evaluation by oxidation of 2,7-dichlorophluorescein diacetate (DCFH-DA) upon incubation of Hepa-RG cells with exosomes, only or in combination with transfection complex. Negative control (CTR) was untreated cells, while positive CTR was cells treated with H2O2. *P < 0.005 vs negative control.
