Table 1 Key resources.

Rabbit polyclonal anti-PCSK9 (IHC: 1:200, WB: 1:1000)

ABclonal

Cat# A7860, RRID:AB_2770818

Rabbit polyclonal anti-LDLR (IHC: 1:150, WB: 1:1000)

Abmart

Cat# T55235; N/A

Rabbit polyclonal anti-NLRP3 (IHC: 1:150, WB: 1:1000)

ABclonal

Cat# A5652, RRID:AB_ 2766412

Rabbit polyclonal anti-caspase-1 (WB: 1:1000)

ABclonal

Cat# A0964, RRID:AB_2757485

Rabbit polyclonal anti-total and cleaved caspase-1 antibody (IHC: 1:150)

Abmart

Cat# PY10200; N/A

Rabbit monoclonal anti-GSDMD (IHC: 1:500, WB: 1:1000)

Abcam

Cat# ab219800, RRID:AB_2888940

Rabbit polyclonal anti-IL-1β (IHC: 1:150, WB: 1:1000)

ABclonal

Cat# A16288, RRID:AB_2769945

Rabbit polyclonal anti-IL-18 (IHC: 1:150, WB: 1:1000)

Proteintech

Cat# 10663-1-AP, RRID:AB_2123636

Rabbit polyclonal anti-StAR (IHC: 1:150, WB: 1:1000)

Immunoway

Cat# YN1369; N/A

Rabbit polyclonal anti-CYP11a1 (IHC: 1:150, WB: 1:1000)

Abmart

Cat# PU986898; N/A

Rabbit polyclonal anti-3β-HSD (IHC: 1:200, WB: 1:1000)

Immunoway

Cat# YN0349; N/A

Rabbit polyclonal anti-3β-tubulin (WB: 1:5000)

ABclonal

Cat# AC015, RRID:AB_2773007

Goat anti-rabbit IgG (H + L), HRP conjugate (WB: 1:1000)

Proteintech

Cat# SA00001-2, RRID:AB_2722564

Goat Anti-Rabbit IgG(H + L), biotin conjugate (IHC: 1:200)

Proteintech

Cat# SA00004-2, RRID:AB_2890946

High-fat-diet

Beijing Keao Xieli Feed

Cat# D12492

Pyrroloquinoline quinone

Cima Science

Cat# 72909-34-3

Palmitic acid

Kunchuang Biotechnology

Cat# SYSJ-KJ

MCC950

SparikJade

Cat# SJ-MX0058A

DMEM/F-12

Gibco

Cat# C11330500BT

Certified fetal bovine serum

VivaCell Bioscience

Cat# C04001-500

Horse Serum

VivaCell Bioscience

Cat# C2510-0500

Trypsin-EDTA solution, 0.25% (without phenol red)

Solarbio

Cat# T1300

OCT compound

Tissue Tek

Cat# 4583

Oil Red O

Sigma-Aldrich

Cat# 1320-06-5

Western blotting stripping buffer

Solarbio

Cat# SW3020

Propidium iodide staining solution

BD, Biosciences

Cat# 51-66211E

Annexin V binding buffer, 10X conc

BD, Biosciences

Cat# 51-66121E

DNase/RNase-free water

Solarbio

Cat# R1600

Trizol reagent

Solarbio

Cat# 15596-018

Triton X-100

Sigma-Aldrich

Cat# V900502

Tween 20

Sigma-Aldrich

Cat# V90054

Phosphate-buffered saline

Thermo Scientific

Cat# 20012027

Total cholesterol assay kit

Jiancheng (Nanjing, China)

Cat# A111-1-1

Triglyceride assay kit

Jiancheng (Nanjing, China)

Cat# A110-1-1

Low-density lipoprotein cholesterol assay kit

Jiancheng (Nanjing, China)

Cat# A113-1-1

High-density lipoprotein cholesterol assay kit

Jiancheng (Nanjing, China)

Cat# A112-1-1

Testosterone assay kit

Jiancheng (Nanjing, China)

Cat# H090-1-2

BCA protein assay kit

Thermo Scientific

Cat# 23227

SDS-PAGE gel kit

Solarbio

Cat# P1200

eECL western blotting kit

ComWin Biotech

Cat# CW0049M

DAB substrate kit (20X)

Solarbio

Cat# DA1010

One-step gDNA removal and cDNA synthesis SuperMix

TransGen Biotech

Cat# AT311-03

ChamQ universal SYBR qPCR master mix

Vazyme (Nanjing, China)

Cat# Q711-02

LDH cytotoxicity assay kit

Beyotime (Hangzhou, China)

Cat# C0016

Cell counting kit-8 (CCK8)

Biosharp

Cat# BS350A

riboFECT CP transfection kit

RiboBio

Cat# C10511-1

C57BL/6 J male mice

Laboratory Animal Center of the University of South China

N/A

TM3

Cellcook Biotech (Guangzhou, China)

Cat# CC9048

See Table S1 for qRT-PCR primers

This paper

N/A

ZEN

Carl Zeiss

https://www.zeiss.co.jp/microscopy/ products/microscopesoftware/zen.html

ImageJ

NIH

https://imagej.nih.gov/ij/

ACDsee

ACD Systems International Inc

https://www.acdsee.cn

MetaboAnalyst 4.0

-

https://www.metaboanalyst.ca/MetaboAnalyst/faces/home.xhtml

R

MathSoft

https://www.r-project.org

Compound Discoverer 3.1 (CD 3.1) software

Thermo Fisher Scientific Inc.

-

GraphPad Prism 9

GraphPad Software

https://www.graphpad.com/

Glass slide

Citoglas

Cat# 7105 P

PVDF transfer membrane

Merck Millipore

Cat# GVWP02500