Fig. 6: UNC0379+adavosertib combination induces cell death through mitotic catastrophe in glioblastoma primary cells. | Cell Death & Disease

Fig. 6: UNC0379+adavosertib combination induces cell death through mitotic catastrophe in glioblastoma primary cells.

From: Targeted inhibition of the methyltransferase SETD8 synergizes with the Wee1 inhibitor adavosertib in restraining glioblastoma growth

Fig. 6: UNC0379+adavosertib combination induces cell death through mitotic catastrophe in glioblastoma primary cells.

a Each primary cell line (GB-1, GB-2, and GB-3) was treated with 5 μM UNC0379 and 400 nM adavosertib, alone or in combination, for 48 h and compared with vehicle-treated control cells (CTRL). Cells viability was assessed using the MTT assay. Statistical analyses were performed using one-way ANOVA, followed by multiple comparison. p value was referred to CTRL. **p < 0.01; *p < 0.05. b Cell cycle distribution of cells, treated as in (a), was monitored by FACS analysis. Percentage of cells in G1/S and G2/M is indicated. All values are given as mean ± standard deviation of at least three replicates. Statistical analyses were performed using one-way ANOVA, followed by multiple comparison. In panel we indicated the one-way ANOVA p value. p value was referred to G1/S and G2/M in CTRL cells. ***p < 0.001; *p < 0.05. c Each primary cell line was treated with 5 μM UNC0379 and 400 nM adavosertib, alone or in combination, for 72 h, and compared with vehicle-treated control cells. Death of cells was monitored by detecting caspase activity. To inhibit caspase activity, cells were also incubated with z-VAD-FMK (+ z-VAD) from the time of UNC0379+adavosertib addition. All values are given as mean ± standard deviation of at least three replicates. Statistical analyses were performed using one-way ANOVA, followed by multiple comparison. p value was referred to CTRL. ***p < 0.001; *p < 0.05.

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