Fig. 1: FAO confers tamoxifen resistance in ER-positive BC cells. | Cell Death & Disease

Fig. 1: FAO confers tamoxifen resistance in ER-positive BC cells.

From: Targeting c-Jun inhibits fatty acid oxidation to overcome tamoxifen resistance in estrogen receptor-positive breast cancer

Fig. 1: FAO confers tamoxifen resistance in ER-positive BC cells.The alternative text for this image may have been generated using AI.

a Venn diagram of overlapping genes significantly upregulated in tamoxifen-resistant (TamR) MCF7 and T47D cells identified in the GEO dataset GSE144378. b Gene Ontology analysis of the significantly enriched biological processes for 334 upregulated genes in both MCF7-TamR and T47D-TamR cells. c–e Comparison of CPT1 enzymatic activities (c), fatty acid oxidation (FAO) rates (d), and cellular ATP levels (e) between TamR and parental MCF7 and T47D cells. f, g RT-qPCR and western blot assessment of the mRNA (f) and protein (g) levels of CPT1A, CPT1B, and CPT1C in TamR and parental MCF7 and T47D cells. h Representative images for immunofluorescence analysis of CPT1A (green) in TamR and parental MCF7 cells. DAPI (blue) served as a marker for nuclei. i Comparison of CPT1 enzymatic activities in MCF7-TamR cells with or without etomoxir (ETX) treatment for 24 h. j CCK-8 analysis of MCF7-TamR cells treated with a concentration gradient of tamoxifen combined with or without ETX for 72 h. Unpaired Student’s t test in (c–f, i); ***P < 0.001.

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