Fig. 3: c-Jun activates FAO to induce tamoxifen resistance. | Cell Death & Disease

Fig. 3: c-Jun activates FAO to induce tamoxifen resistance.

From: Targeting c-Jun inhibits fatty acid oxidation to overcome tamoxifen resistance in estrogen receptor-positive breast cancer

Fig. 3

a Western blot analysis to detect the c-Jun overexpression in both wild-type MCF7 and T47D cells. b–d Comparison of CPT1 enzymatic activities (b), FAO rates (c), and cellular ATP levels (d) in wild-type MCF7 and T47D cells transfected with c-Jun overexpressing construct or empty vector. e CCK-8 analysis of c-Jun or empty vector transfected wild-type MCF7 and T47D cells which were treated with a concentration gradient of tamoxifen for 72 h. f CPT1 enzymatic activities in c-Jun-overexpressing wild-type MCF7 cells with or without ETX treatment for 24 h. g CCK-8 analysis of c-Jun-overexpressing wild-type MCF7 cells treated with a concentration gradient of tamoxifen combined with or without ETX for 72 h. Unpaired Student’s t test in (b–d, f); **P < 0.01, ***P < 0.001.

Back to article page