Fig. 4: c-Jun recruits the CBP/P300 to activate CPT1A transcription.

a, b Western blot and RT-qPCR assessment of the protein (a) and mRNA (b) levels of CPT1A in wild-type MCF7 and T47D cells with or without c-Jun knockout. c Schematic diagram of the TRE motif location on the wild-type (WT) or mutant (MUT) promoter of CPT1A for luciferase reporter assays. TRE, TPA-responsive element. d HEK293T cells were transfected with empty vector or c-Jun-overexpressing plasmid to analyse the luciferase reporter activity driven by the WT or MUT CPT1A promoter. e Chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) analysis of c-Jun occupancy on the CPT1A promoter region around the TRE motif in TamR and parental MCF7 and T47D cells. f STRING analysis of proteins interact with c-Jun. g Immunoprecipitation assay to confirm the interaction between c-Jun and CBP/P300 in T47D cells. h ChIP-qPCR assessment for the comparison of CBP, P300, and H3K27ac occupancy on the CPT1A promoter in MCF7 cells with or without c-Jun knockout. i HEK293T cells were transfected with empty vector, c-Jun-, CBP-, or P300-overexpressing plasmid alone or together as indicated to analyse the luciferase reporter activity driven by the WT CPT1A promoter. Unpaired Student’s t test in (b, e, h), and paired Student’s t test in (d, i); **P < 0.01, ***P < 0.001, n.s., not significant.