Fig. 1: HSP90α transcript and protein abundance correlate with the SIRT1 protein level in DLBCL cell lines. | Cell Death & Disease

Fig. 1: HSP90α transcript and protein abundance correlate with the SIRT1 protein level in DLBCL cell lines.

From: SIRT1 and HSP90α feed-forward circuit safeguards chromosome segregation integrity in diffuse large B cell lymphomas

Fig. 1

A HSP90α (left) and SIRT1 (right) protein levels in a panel of OxPhos- dependent (OxPhos-dep) and -independent (OxPhos-indep) DLBCL cell lines. Upper panels show representative immunoblots from three independent experiments; GAPDH was used as a loading control. Lower panels represent relative quantifications of band intensities from digital images. Relative HSP90α and SIRT1 protein expression was calculated as a ratio of HSP90α or SIRT1 band intensity to GAPDH band intensity. The individual values were normalized to the averaged value obtained for all analyzed DLBCL cell lines, which was assigned as arbitrary value 1. B Transcript abundance for HSP90α (left) and SIRT1 (right) in OxPhos- dependent and -independent DLBCL cell lines. Relative abundance of HSP90α and SIRT1 transcripts was determined using 2−ΔΔCT method, with 18S ribosomal RNA used as a reference gene. The individual values were normalized to the average obtained from all investigated cell lines, which was assigned as value 1. In (A) and (B) bars represent averages from 3 independent experiments ± standard deviation (SD). Statistical comparisons were performed using unpaired t test, * indicates p < 0.05. C The correlation between HSP90α transcript (left) and protein level (right) with the SIRT1 protein abundance; r - the Spearman correlation coefficient.

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